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The Effect of 1-Propanol on the Rotational Mobility of n-(9-Anthroyloxy) stearic acid in Outer Monolayers of Neuronal and Model Membranes KCI 등재후보
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- URLhttps://db.koreascholar.com/Article/Detail/341001
The aim of this study was to provide a basis for the molecular mechanism underlying the pharmacological action of ethanol. We studied the effects of 1-propanol on the location of n-(9-anthroyloxy)palmitic acid or stearic acid (n-AS) within the phospholipids of synaptosomal plasma membrane vesicles (SPMV). The SPMV were isolated from the bovine cerebral cortex and liposomes of total lipids (SPMVTL) and phospholipids (SPMVPL). 1-Propanol increased the rotational mobility of inner hydrocarbons, while decreasing the mobility of membrane interface, in native and model membranes. The degree of rotational mobility varied with the number of carbon atoms at positions 16, 12, 9, 6 and 2 in the aliphatic chain of phospholipids in the neuronal and model membranes. The sensitivity of increasing or decreasing rotational mobility of hydrocarbon interior or surface by 1-propanol varied with the neuronal and model membranes in the following order: SPMV, SPMVPL and SPMVTL.
Materials and Methods
Materials
SPMV preparation
Liposome preparation
Fluorescence measurements
Results
Ordering effects of 1-propanol on the rotationalmobility of the membrane interface
Disordering effects of 1-propanol on the rotationalmobility of the hydrocarbon interior
Discussion
Acknowledgments
Conflict of interest
References
