Interferon-stimulated gene 15 (ISG15) is known to interfere with viral replication and infection by limiting the viral infection of cells. Interferon-stimulated gene 15 (ISG15) interferes with viral replication and infectivity by limiting viral infection in cells. It also plays an important role in the immune response. In this study, tissue-specific expression of ISG15 in healthy rock bream samples and spatial and temporal expression analysis of rock bream ISG15 (RbISG15) were performed following rock bream iridovirus (RSIV) infection. RbISG15 expression was significantly higher in the eye, gill, intestine, kidney, liver, muscle, spleen, and stomach, but low in the brain. There were particularly high levels of expression in the liver and muscle. RbISG15 expression was also examined in several tissues and at various times following RSIV infection. ISG15 expression increased within 3 h in the whole body and decreased at 24 h after infection. In addition, temporal expression of several tissues following RSIV infection showed a similar pattern in the muscle, kidney, and spleen, increasing at 3 h and decreasing at 72 h. These results suggest that ISG15 plays an important role in the immune response of rock bream. Overall, this study characterizes the response of RbISG15 following RSIV infection.

ABSTRACT
  INTRODUCTION
  MATERIALS AND METHODS
  RESULTS
  DISCUSSIONS
  REFERENCES

• Kyung-Hee Kim(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• In Jung Yang(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• Woo-Jin Kim(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• Choul-Ji Park(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• Jong-Won Park(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• Gyeong Eon Noh(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• Seunghyung Lee(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• Young Mee Lee(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• Hyung Kyu Hwang(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
• Hyun Chul Kim(Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) Corresponding Author