Koi herpesvirus (KHV), also known as Cyprinid herpes virus 3 (Cyprinid 3) is lethal disease in common carp and koi (Cyprinus carpio). Two different groups (KK and RK) were infected KHV by intraperitoneal injection. Fish for gene expression analysis were sampled at 0 h, 12 h, 24 h, 48 h and 72 h post infection (p.i). The results showed that two immune related gene, Interferons (INFs) ɑβ and Interleukin (IL)-12 p35 induced a high response in RK. The IL-12 p35 cytokine and Toll-like receptor (TLR) 9 were significantly high expressed on 48 h post infection (p.i) in RK as compared to the KK. The histopatological examination reveals focal necrosis in liver and infiltrate of lymphocytes in spleen of KK as compared to the RK. In immunohistochemistry analysis, the KHV protein high expressed in the infected kidney cell and slenocyte of KK. Therefore, the expression of IL-12 p35, IFN ɑβ and TLR 9 may provide a potentially genes related with KHV resistance in Koi and red common carp × koi.

ABSTRACT
  INTRODUCTION
  MATERIALS AND METHODS
  RESULTS
  DISCUSSIONS
  REFERENCES

• Ju-ae Hwang(Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
• Jung Eun Kim(Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
• Hyeong-su Kim(Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
• Jeong-Ho Lee(Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS)) Corresponding Author