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탈질미생물을 이용한 질산성 질소의 산소 및 질소 동위원소 분석법 소개 KCI 등재
Introduction of Denitrification Method for Nitrogen and Oxygen Stable Isotopes (δ15N-NO3 and δ18O-NO3) in Nitrate and Case Study for Tracing Nitrogen Source
pp.459-469
Nitrogen (N) loading from domestic, agricultural and industrial sources can lead to excessive growth of macrophytes or phytoplankton in aquatic environment. Many studies have used stable isotope ratios to identify anthropogenic nitrogen in aquatic systems as a useful method for studying nitrogen cycle. In this study to evaluate the precision and accuracy of denitrification bacteria method (Pseudomonas chlororaphis ssp. Aureofaciens (ATCC® 13985)), three reference (IAEA-NO-3 (Potassium nitrate KNO3), USGS34 (Potassium nitrate KNO3), USGS35 (Sodium nitrate KNO3)) were analyzed 5 times repeatedly. Measured the δ15N-NO3 and δ18O-NO3 values of IAEA-NO-3, USGS 34 and USGS35 were δ15N: 4.7±0.1‰ δ18O: 25.6±0.5‰, δ15N: -1.8±0.1‰ δ18O: -27.8±0.4‰, and δ15N: 2.7±0.2‰ δ18O: 57.5±0.7‰, respectively, which are within recommended values of analytical uncertainties. Also, we investigated isotope values of potential nitrogen source (soil, synthetic fertilizer and organic-animal manures) and temporal patterns of δ15N-NO3 and δ18O-NO3 values in river samples during from May to December. δ15N-NO3 and δ18O-NO3 values are enriched in December suggesting that organic-animal manures should be one of the main N sources in those areas. The current study clarifies the reliability of denitrification bacteria method and the usefulness of stable isotopic techniques to trace the anthropogenic nitrogen source in freshwater ecosystem.
재료 및 방 법
1. 미생물 (Pseudomonas chlororaphis ssp.aureofaciens) 선택
2. 미생물 배양
3. 미생물을 이용한 탈질산화 과정
4. 미량가스 동위원소 분석 과정
5. 안정동위원소 표준시료의 조제
6. 시료농도 및 배양시간
7. 안정동위원소비 분석
8. 환경시료에의 적용
결과 및 고 찰
1. 시료 농도 변화에 따른 안정동위원소비의 정밀 · 정확도연구
2. 탈질 박테리아 배양시간 변화에 따른 안정동위원소비의정밀 · 정확도연구
3. 지하수에서의 질소기원 유기물 변동 연구
적 요
사 사
REFERENCES
