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Bioprocessed polysaccharide from Lentinus edodes mycelia cultures with turmeric represses the proinflammatory cytokine expression of the porcine macrophage infected by Salmonella Choleraesuis KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/341599
pp.150-155
Turmeric is known for its ability to enhance immunity via anti-inflammatory and anti-oxidant effects. Salmonella enterica species contain a large number of pathogenic serotypes that are adapted to a broad range of vertebrate hosts. Our previous study revealed that bioprocessed polysaccharides from the liquid culture Lentinus edodes fungal mycelia containing turmeric (BPP-turmeric) is able to alter chicken macrophage responses and increases chick survival against Salmonella enterica infection. In this study, we examined the immunomodulatory effects of BPP-turmeric on the porcine macrophage 3D4/31 cell line infected with Salmonella enterica subsp. enterica serovar Choleraesuis (S. Choleraesuis) or S. Enteritidis. Our experimental analyses demonstrated that BPP-turmeric (i) does not alter phagocytic and killing activity of 3D4/31 against either Salmonella serotypes, but that it (ii) represses mRNA transcription of interleukin (IL)-6, IL-8, and tumor necrosis factor α in response to Salmonella infection. Collectively, these results imply that BPP-turmeric has an immunomodulatory effect that represses pro-inflammatory cytokine expression in porcine macrophages, suggesting that it may protect swine from salmonellosis via controlling Salmonella-induced hyperinflammation.
MATERIALS AND METHODS
Bacterial strains and macrophage cell line
BPP-turmeric preparation
Salmonella phagocytosis and killing assay
Cytokine expression assay
RESULTS
BPP-turmeric does not significantly affect phagocytosis and killing of S. Choleraesuis or S. Enteritidis by porcine macrophages
BPP-turmeric represses cytokine expression in Salmonella-infected3D4/31 macrophages
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
