D_(10) values obtained for radiation alone in Bacillus subtilis and Clostridium perfrigenes were 0.35-0.48 kGy in vegetative cells, and 2-2.08 kGy in spores, respectively. Irradiation dose of 24 kGy completely inhibited spores. In the case of heat treatment, D_(50, 60) values ranged from 10 to 14 minutes in vegetative cells, and D_(70, 80, 90), values ranged from 10 to 140 minutes in spores. In the case of combined treatment with heat and radiation, D_(10) values ranged from 1 to 1.25 kGy in vegetative cells, and from 3.42 to 3.61 kGy in spores. Thus, resistance of cells to gamma radiation did not seem to be influenced by pre-heating.