The biodegradation of high concentration of benzoate by enrichment culture with Pseudomonas sp. was investigated. During 50 days continuous culture, average of removal rate of benzoate and COD were 90% and 83%, respectively. And the enzymatic activity of catechal 2,3-dioxygenase was determined in the continuous culture but not Catechol 1,2-dioxygenase. On the other hand, Pseudomonas sp in the culture was investigated with SEM and the result was revealed that the cell shape was more demage according the higher concentration of benzoate.

• 정준영(충북대학교 식품공학과) | Jun-Young Jeong
• 김교창(충북대학교 식품공학과) | Kyo-Chang Kim