Effects of benzoate (0-0.6 g/l and sorbate (0-2.0 g/l) on the growth of Escherichia coli O157:H7 in tryptic soy broth at various pH levels (4-8) and temperatures (4℃, 37℃) were investigated. Benzoate and sorbate were inhibited the growth of E. coli 0157:H7 up to 12 hours cultivation at 4℃, and 2.0 g/l sorbate was only inhibited during 48 hours cultivation at 37℃. Among the pH levels tested, pH 4 showed significant inhibitory effect against the E. coli O157:H7 on 4℃ and at 37℃, respectively. When used in combination 0.2 g/l benzoate and sorbate were completely inhibited the growth of E. coli O157:H7 on pH 4 and at 37℃. While on pH 5 at 4℃, all of the concentration tested did not exert any inhibitory effect. The combined effects were retarded more than single treatment of E. coli O157:H7.