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pp.181-187
Lactobacillus acidophilus NCFM, Lactobacillus casei YIT 9018, Bifidobacterium longum 8001, and Bifadobacterium longum 8025 at the level of 10^6 cfu/ml were cultured with 10⁴ cfu/ml of Escherichia colt O157:H7 KSC 109 or Salmonella typhimurium ATCC 14028, in order to verify the effects of lactic acid bacteria and bifidobacteria on the growth of the pathogens. In the mixed culture of lactic acid bacteria with E. colt O157:H7 KSC 109, growth inhibition and atypical microcolonies of E. colt O157:H7 KSC 109 were observed. The pathogens inoculated grew for 5 hours (pH 5.3), by the time L. acidophilus NCFM reached the exponential growth phase, and then the surviving pathogens were decreased to 10¹ cfu/ml after 35 hours. When L. casei YIT 9018 was grown with the pathogens, they grew for 10 hours (pH 4.6), by the time L. casei YIT 9018 reached the end of exponential growth phase, and then the surviving pathogens were decreased drastically. Up to the stationary growth phase of lactic acid bacteria, L. acidophilus NCFM exhibited stronger inhibition against the pathogens than L. casei YIT 9018 did, which might be attributed to its faster growth. Likewise bifidobacteria inhibited the growth of the pathogens. tested, bifidobaceria was weaker in the inhibitory activity than lactic acid bacteria. When Bifidobacterium longum 8001 was cultured with the pathogens, E. colt O157:H7 KSC 109 was gradually inhibited at the stationary growth phase of bifidobacteria, atypical microcolonies were formed on Levine EMB medium after 48 hours, and Salmonella grew up to 10^6 cfu/ml, then was drastically inhibited at the exponential growth phage of Bifxdobacterium longum 8001. But when Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same level of Bifidobacteriuam longum 8025 after 10 hours, then the surviving pathogens were decreased drastically.
