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pp.419-424
An enzyme-linked immunosorbent assay (ELISA) was established for the detection of zearalenone by using monoclonal antibodies produced by Z-M-26 hybridoma cells when injected into a mouse and zearalenone-oxime-OVA conjugate. Zearalenone-oxime-OVA conjugates were diluted with carbonyl buffer, coated to 96 well microtiter plates at 4℃ overnight and blocked with 1% BSA overnight. One thousand times diluted antibody solution together with standard zearalenone or sample was added to 96-well microtiter plates and stood overnight. A secondary antibody conjugated with HRP was added and an hour later, enzyme substrate (TMBZ) solution was added for color develpment. After 30 minutes, coloring reaction was terminated by adding 2 N H₂SO₄ and the O.D. was measured at 450 nm. Detection range of this method was about 0.1-100 ppb. The established indirect competitive ELISA method was suitable for a rapid and effective analysis of zearalenone in agricultural products.
