Effects of substrates, non-aggregated medium and Rootone on the rooting of spearmint (Mentha spicata), peppermint (Mentha x piperit) and catmint (catnip:Nepeta cataria) were investigate and results were as follow. Five types of substrate (saproilite (S), peatmoss (P), S:P 75:25, S:P 50:50, S:P 25:75v/v) were used as rooting media. In the rooting rate and number of roots, peppermint and catmint had higher values in saprolite with significant, but spearmint was shown the better result in S:P 25:75 mixed substrate than saprolite without significances. Root length of spearmint was promoted in mixed substrate of S:P 50:50 and 75;25, and catmint was better in saprolite substrate. Rooting rate and number of roots were shown more decreased in single peatmoss substrate and mixed substrate than saproilte. The effects of aggregated medium (S) and non aggregated medium (tap water) on the rooting of different stem part of mints were tested. Rooting and number of rooting of spearmint and peppermint in water medium were more increased than in saprolite medium. But catmint was not rooted in any medium. Rooting of upper parts with growing points was more increased than under part of stem, which was relative hardy. In Rootone treatments test on rooting of stem cuttings, all treatments very strongly promoted the rooting and number of roots not only in upper stems but also in under part of stem in saprolite and water medium. Rootone treatments (drying 10 minutes after dipping on wetted stem) was very effective in water medium. Especially catmint treated with Rootone promoted a root formation in water medium This research was clarified the possibilities of rooting on spearmint, peppermint, and catmint cuttings in water medium, when especially treated with Rootone.