This study was designed to investigate the effects of multi-enzyme on diarrhea and immune responses of weaned pigs. A total 36 weaned pigs (5.92 ± 0.48 kg BW; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/ treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multienzyme (Multi; mixture of β-mannanase, xylanase, α-amylase, protease, β-glucanase, and pectinase). Pigs were fed their respective diets for 6 wk. Frequency of diarrhea, levels of packed cell volume (PCV), white blood cells (WBC), immunoglobulins, cortisol, tumor necrosis factor-α (TNF-α), transforming growth factor- β (TGF-β), and C-reactive protein (CRP) were measured. Multi group tended to decrease (p<0.1) diarrhea frequency than CON group during 2 wk after weaning. Lower values of PCV on d 3 (p<0.05) and d 7 (p<0.1) were found in Multi group compared with CON group. There were no significant differences on WBC number and immunoglobulin (Ig) M and A between Multi and CON groups. However, Multi group tended to increase (p<0.1) Ig G on d 7 than CON group. Moreover, Multi group showed modulated immune responses, indicated by decreased levels of cortisol (p<0.05) on d 7 and 14, TNF-α on d 3 (p<0.05) and d 7 (p<0.10), TGF- β on d 2 (p<0.05) and d 7 (p<0.10), and CRP (p<0.10) on d 3 and 7 after weaning compared with CON group. Consequently, inclusion of multi-enzyme in diets for weaned pigs improved gut health and modulated immune responses of weaned pigs.

Ⅰ. Introduction
 Ⅱ. Materials and Methods
  1. Experimental design, animals, and diets
  2. Sample collection and analyses
 Ⅲ. Results and Discussion
 References

• Min, Ye-Jin(National Institute of Animal Science, Rural Development Administration) | 민예진
• Kim, Jun-Su(Department of Animal Science and Biotechnology, Chungnam National University) | 김준수
• Kim, Sheen-A(Department of Animal Science and Biotechnology, Chungnam National University) | 김신아
• Jang, Ki-Beom(Department of Animal Science and Biotechnology, Chungnam National University) | 장기범
• Mun, Da-Ye(Department of Animal Science and Biotechnology, Chungnam National University) | 문다예
• Kim, Byeong-Hyeon(Department of Animal Science and Biotechnology, Chungnam National University) | 김병현
• Choe, Jee-Hwan(Department of Animal Science and Biotechnology, Chungnam National University) | 최지환 Corresponding author
• Song, Min-Ho(Department of Animal Science and Biotechnology, Chungnam National University) | 송민호