National Institute of Environmental Research (NIER) has proposed new methodology for estimating unit load in order to overcome the limitations of past unit load based on short-term and local area based data. In the case of agricultural land, however, the results presented by NIER are still limited because of various agricultural activities and farmland characteristics. In this study, liquid manure treated paddy field and organic farming upland were selected for considering agricultural diversity. Four different methods for evaluating unit load were used for comparing previous research results. The results of different methods presented various trends compared with those of existing studies. Paddy field treated liquid manure was 1.3 times higher for T-N load and 3.1 times for T-P load than conventional paddy field. Conventional upland was 4.4 times higher for T-N load and 1.8 times higher for T-P load than organic farming upland. In the case of non-conventional farmland, this study showed different values with the unit loads presented by NIER. This implies that it is necessary to review and apply the unit loads considering various agricultural conditions when establishing environmental policy and rural planning.