Background: Angelica gigas, A. sinensis, and A. acutiloba are commercially important in the herbal medicine market, and among them, A. gigas has the highest economic value and price. However, their similar morphological traits are often used for fraud. Despite their importance in herbal medicine, recognition of the differences between Angelica species is currently inadequate. Methods and Results: A multiplex polymerase chain reaction (PCR) method was developed for direct detection and identification of A. gigas, A. sinensis, and A. acutiloba. The gene for the distinction of species was targeted at ITS in the nucleus and trnC-petN gene in chloroplasts. The optimized multiplex PCR in the present study utilized each Angelica species-specific primer pairs. Each primer pair yielded products of 229 base pairs (bp) for A. gigas, 53 bp for A. sinensis, 170 bp for A. acutiloba. Additionally non-specific PCR products were not detected in similar species by species-specific primers. Conclusions: In the present study, a multiplex-PCR assay, successfully assessed the authenticity of Angelica species (A. gigas, A. sinensis, and A. acutiloba). and whole genome amplification (WGA) was performed after DNA extraction to identify, the species in the product. The detection method of raw materials developed in the present study could be applied to herbal medicine and health functional food management.

서 언
 재료 및 방법
  1. 표준 시료확보 및 전처리
  2. 유전자 추출 및 유전자 증폭
  3. 종 특이 프라이머 탐색
  4. Multiplex PCR
 결 과
  1. 종 특이 프라이머의 설계 및 PCR 조건 최적화
  2. 가공 식품에 대한 적용
 고 찰
 REFERENCES

• 김용상(콜마비앤에이치 식품과학연구소, Food Science R&D Center, Kolmar BNH, Sejong 27670, Korea.) | Yong Sang Kim
• 박혁주(콜마비앤에이치 식품과학연구소, Food Science R&D Center, Kolmar BNH, Sejong 27670, Korea.) | Hyeok Joo Park
• 이동희(경북바이오산업연구원 식품시험검사사업단, Gyeongbuk Institute for Bio Industry Food Testing and Inspection Project, Andong 36728, Korea) | Dong Hee Lee
• 김현규(콜마비앤에이치 식품과학연구소, Food Science R&D Center, Kolmar BNH, Sejong 27670, Korea.) | Hyun Kyu Kim Corresponding author