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pp.55-63
Background: The present study assessed the response of kenaf (Hibiscus cannabinus L., Jangdae) seed to NaCl and the effects of polyethylene glycol (PEG) on kenaf seed germination and vigor. Methods and Results: Seed germination ranged from 11.3% to 58.8% after 24 hours of immersion in NaCl concentrations from 0% to 0.5%. The priming treatments had lower electrical conductivity (EC) values for the seeds than for the control and a deteriorated palisade layer. Priming in 10% PEG for 48 hours increased the germination upto 96.3% in H2O solution and 98.8% in 0.3% NaCl solution compared to that of the control (78.8%). Germination synchronization, and shoot and root growth of the primed seeds were greater than those of the control. The T50 of the control in H2O and 0.3% NaCl solution was 22 and 28 times, respectively. After priming, nine times was sufficient to reach T50 in both solution. The mean number of days to germination (MDG) decreased from 1.43 days for the control to 0.55 days for 0% PEG in H2O solution and from 1.57 days for the control to 0.56 days for 0% PEG in 0.3% NaCl solution. The dry weight after the 10% PEG treatment was higher than that of the control. Conclusions: Taken together, 10% PEG treatment for 24 hours is recommended for kenaf seed invigoration before planting.
MATERIALS AND METHODS
1. Seed and other materials preparation
2. Measurement of seed water uptake and electricalconductivity value
3. Germination tests to NaCl and PEG
4. Measurement of growth degree of shoot and rootto salt
5. Germination performance measurements
6. Scanning electron microscope image
7. Statistics
RESULTS AND DISCUSSION
1. Response of kenaf to NaCl
2. Effect of various immersion times on seed wateruptake
3. Effect of various PEG and immersion times on ionleachate
4. Effect of PEG on surging the germination percentage
5. Effect of PEG on surging both T50 and MDG
6. Effect of PEG on the dry weight
ACKNOWLEDGEMENTS
REFERENCES
