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H9c2 심근세포에서 제주모시풀(Boehmeria quelpaertense)로부터 분리된 flavonoids의 H2O2로 유도된 독성 보호 효과 KCI 등재

Protective Effects of Flavonoids from the Boehmeria quelpaertense against H2O2-Induced Cytotoxicity in H9c2 Cardiomyoblast Cells

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한국자원식물학회지 (Korean journal of plant resources)
한국자원식물학회 (The Plant Resources Society Of Korea)
초록

As a part of an infrastructure project on medicinal herb-based remedies, we conducted a phytohemical investigation of the 100% MeOH extract from the aerial part of Boehmeria quelpaertense; our findings resulted in the isolation of flavonoids (1-2), isoquercitrin (1) and hyperoside (2). The identification and structural elucidation of these compounds were based on 1H-,13C-NMR, and LC ESI IT-TOF MS data. All the compounds isolated from this plant were reported for the first time. In this study, we examined the antioxidant activity of the 1 and 2 on the hydrogen peroxide (H2O2)-induced oxidative stress in a Rat Cardiomyoblast cell line (H9c2). The pretreatment of the flavonoids showed that it protects against H2O2-mediated cell death in the H9c2 cell line. Also, it decreases the intracellular reactive oxygen species (ROS) levels by the flavonoids in the H2O2-treated H9c2 cell line. These results showed that the 1 and 2 are a source of antioxidants. As a result, they might be helpful in preventing the progress of various oxidative stress mediated diseases, including myocardial infarction.

목차
서 언
 재료 및 방법
  시료 추출 및 제조
  시약 및 기기
  추출 및 분획
  n-BuOH 분획으로부터 화합물의 분리
  MeOH 추출물 LC-MS분석
  세포배양
  세포독성
  H2O2에 의한 세포의 손상으로부터 심장세포보호 효과 측정
  유세포분석기(Flow cytometry)를 이용한 H2O2에 유도된 심장 세포내 활성산소종 감소 효과 측정
 결과 및 고찰
  화합물 분리 및 구조 분석
  제주모시풀 MeOH LC-MS 분석
  화합물 1과 2의 H9c2세포에서의 세포생존율에 미치는 영향
  화합물 1과 2의 H9c2 cells에서 H2O2로 유도된 독성 보호효과
  화합물 1과 2의 H9c2세포에서 H2O2로 유도된 세포내 활성 산소종(ROS)에 대한 영향
 적 요
 사 사
 References
저자
  • 조현우(한약진흥재단 한약자원본부 한약재연구팀) | Hyun-Woo Cho 교신저자
  • 박종혁((주)루코) | Jong Hyuk Park
  • 함성호(한약진흥재단 한약자원본부 한약재연구팀) | Seong-Ho Ham
  • 안병관(한약진흥재단 한약자원본부 한약재연구팀) | Byeongkwan An
  • 정호경(한약진흥재단 한약자원본부 한약재연구팀) | Ho Kyung Jung
  • 박호(한약진흥재단 한약자원본부 한약재연구팀) | Ho Bak
  • 심미옥(한약진흥재단 한약자원본부 한약재연구팀) | Mi-Ok Sim
  • 우경완(한약진흥재단 한약자원본부 한약재연구팀) | Kyeong-Wan Woo