논문 상세보기
pp.16-23
Paeonia japonica is a perennial flowering plant used in traditional medicine therapy. The purpose of this study was to investigate the effect of water extract and solvent fractions obtained from P. japonica on anti-oxidative, anti-thrombin, anti-invasive and pro-apoptotic activities in YD-10B cells, human oral squamous carcinoma cell line. Water fraction revealed the highest extraction yield at 11.44% (w/w). Anti-oxidative activity was the highest in ethyl acetate fraction (85.13%). In the thrombin inhibitory activity test, ethyl fraction was the highest, with a value of 87.54%. Release and activation of MMP-2/pro-MMP-2 ratio in thrombin-treated YD-10B cells were significantly inhibited in the ethyl acetate fraction. At a concentration of 120 ㎍/㎖, water extract and solvent fractions of P. japonica inhibited cell proliferation in YD-10B cells except water fraction. Pro-apoptotic effect on human oral squamous carcinoma cell using the Bax/Bcl-2 ratio analysis was higher in water extract than other fractions. These findings suggest that the ethyl acetate fraction of P. japonica potentiates a promising antioxidant, anti-thrombin and anti-invasive agents.
재료 및 방법
재료 및 시약
백작약 물 추출물과 유기용매 분획물 조제
세포배양
세포독성 측정
전자공여능 측정
트롬빈 저해활성 측정
Reverse transcription-polymerase chain reaction (RT-PCR)
Gelatin Zymography
통계 분석
결과 및 고찰
용매별 분획물의 수득율
항산화 효과
트롬빈 저해활성
세포독성 조사
MMP-2와 MMP-9의 발현 및 활성을 통한 암전이 억제 효과
Bax와 Bcl-2의 발현을 통한 암세포 사멸 효과
적 요
References
