논문 상세보기
pp.195-201
본 연구에서는 국내?외에서 유통되는 건강관련식품 중 성기능 개선 및 효과에 대하여 광고?판매하고 있는 제품들에 대하여 최근 의약품으로 허가된 발기부전치료제인 sildenafil, vardenafil, tadalafil 및 sildenafil의 유사물질인 homosildenafil을 대상으로 정성과 정량을 위한 동시분석법을 개발하고 유통 제품 중의 함량을 분석하여 함유 실태를 파악하고자 하였다. 네 가지 성분을 확인할 수 있는 TLC와 LC/MS 분석방법을 개발하였으며, LC/PDA를 사용한 동시 정량분석법을 개발하고, 건강보조식품 등 건강관련 식품 중 sildenafil 등 4종 물질에 대해 총 35개의 시료를 분석한 결과 sildenafil이 7종에서 0.4mg/g~360.9mg/g, homosildenafil이 7종에서 2.2gm/g~336.0mg/g, tadalafid이 2종에서 각각 19.2mg/g, 429.3mg/g로 검출되었으며, 본 연구에서 개발한 동시분석법은 유통식품에 적용 가능하였다.
An easily available, simultaneous identification/determination procedure for sildenafil, homlsildenafil, tadalafil, vardenafil in adulterated health related foods was established by using a combination of three different analytical methods; thin layer chromatography(TLC), liquid chromatography-mass spectrometry(LC/MS)and high-performance liquid chromatography (HPLC)/photo-diode-array detector. The sample solution for TLC was applied to silica gel 60F_254 plates with ethylacetate/acetonitrile/25%ammonia (90:10:5) as developing solvent. Spots were located under UV radiation at 254 nm and dragendolfs reagent. Mass spectra of the compounds by LC/MS were investigated with electrospray ionization(ESI) interface, under positive ion mode. The HPLC analysis was performed on the column of capcell pak C_18(UG120, 4.6×250mm I.D. 5㎛) with 0.1% sodium 1-hexansulfonate (in 0.1% phosphoric acid)/acetonitrile (73:27) as a mobile phase, and effluent was monitored with a photo-diode-array detector. Quantitative HPLC analysis of the compounds were detected at 291nm. When this procedure was applied to commercial foods, Sildenafil content was in the range of 0.4mg/g~360.9mg/g from 7 out of 35 samples. Homosildenafil content was in the range of 2.2gm/g~336.0mg/g from 7 out of 35 samples. Tadalafil content was 429.3mg/g, 9.6mg/500mg from 2 out of 35 samples. The procedure described here is available for the screening of sildenafil, homosildenafil, tadalafil, vardenafil.
