The purpose of the present study was to investigate differences in the sensitizing potential of Rhus Veniciflua(Rhus-II), when tested by the guinea pig maximization test(GPMT) and Freund's complete adjuvant test(FCAT) with an identical, intradermal induction concentration. A new grading. classification of the sensitization potential is proposed. The GPMT was conducted according to OECD guideline #406, using a multiple-dose design and test results were analysed with logistic regression analysis. During the induction stage, we injected intradermally each three site 0.1 ml( 1mg/animal) test material. 0.1ml complete Freund's adjuvant and 0.1ml the test agent emulsified in the adjuvant. 7 days later, we induced weak sensitization with 10% sodium lauryl sulfate(SLS) and applide 1ml(10mg/animal) test agent topically on the same site and made a tight occlusion. 14 days later we challenged with 1ml(10mg/animal) of test material on the flank and observed ant 24 hours and 48 hours later. The results were also observed 0% at 24 hours challenge. The results observed 48 hours after challenge were the identical. These data indicated that. although Rhus-II is a no contact allergen. It was reported that the skin sensitization by Rhus-II was not detected the skin sensitization in the guinea pig maximization test (GPMT). Consequently, it was confirmed that Rhus-Il had no contact allergic sensitization in guinea pig maximization test.

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