논문 상세보기
pp.236-243
벼멸구 저항성 유전자의 mapping, 벼멸구 저항성 관련 QTL 분석, 주요 농업형질과 벼멸구 저항성과의 관계 분석 등에 관한 연구를 수행 하여 얻은 결과는 다음과 같다. 벼멸구 저항성을 가진 통일형 벼인 '삼강'과 자포니카형 벼인 '낙동'을 교배하여 양성된 F_1의 약을 배양하여 육성된 120 DH 계통을 유전자 지도 작성을 위한 mapping 재료로 이용 하였다. Maker 검정에서 양친에 다양성을 나타낸 SSR 73, STS 89개 등 총 162
This study was conducted to develop a japonica-type rice cultivar with brown planthopper (BPH) resistance using DNA markers. A doubled haploid (DH) population consisting of 120 pure-lines was established by anther culture of F_1 hybrids between 'Samgang', a Tongil type BPH resistance cultivar, and 'Nagdong', a japonica cultivar. To determine the map position of genes responsible for BPH resistance in rice, a genetic map was constructed based on 120 DH lines. A total of 162 molecular markers were classified into 12 linkage groups, covering 1,884 Kosami centimorgan (cM) with an average of 11.6 cM. Five QTLs (qBPR3, qBPR6, qBPR7, qBPR8, and qBPR12) associated with BPH resistance were identified and mapped on chromosomes 3, 6, 7, 8, and 12, respectively, using the genetic map constructed in this study. To analyze the relationship between BPH resistance and agronomic traits, a total of eight QTLs related to the agronomic traits were detected on 12 rice chromosomes. In an analysis of relationships, three QTLs (qBPR3, qBPR7, and qBPR8) showed a linkage with tested agronomic traits. A QTL (qBPR3) located on chromosome 3 (RM282-3023) was closely linked to culm length (qCL3). The QTL (qBPR8) for BPH resistance on the short arm of chromosome 8 also overlapped the region detected in culm length (qCL8).
