논문 상세보기
pp.305-313
We investigate that the impact of freshwater organism exposed to the salinity environment by the frequent rainfall following climate change. To evaluate the stress response following salinity exposure, we assessed the survival rate, molting success rate, the developmental period and mouthpart deformities in Chironomus riparius. In addition, we measured the molecular responses of biomarker gene, gene expression of heat shock protein 70 (HSP70) in C. riparius exposed to salinity after 96 hour. The C. riparius survival rates were showed on time dependent manner and not observed survival organisms above 15 psu at day 4. The pupation and emergence of C. riparius were not seen above 15 psu, and the molting success rate was less than 20% at 10 psu. The developmental retardation of C. riparius was well observed in the pupation and emergence period and was delayed by 4 days at 10 psu compared to the control and 5 psu. The mouthpart deformities after salinity exposure at 96 or 72 hour were observed at 10 psu and 15 psu. The expression of C. riparius HSP70 level was significantly increased exposure to 5 psu and 10 psu. Thus, salinity has been caused to be various ecotoxicological and molecular stress responses on freshwater organisms similar to harmful substances such as EDCs and so on.
서 론
재료 및 방 법
1. 실험 생물
2. 염분 노출 및 조건
3. 하순기절 관찰
4. RNA 추출 및 cDNA 합성
5. Real-Time RT-PCR을 통한 유전자 증폭
6. 계통진화적 분석
7. 통계적 자료 분석
결 과
1. 생존율
2. 탈피율과 발육기간
3. 하순기절 기형 관찰
4. HSP70 계통진화적 분석과 유전자 발현
고 찰
적 요
REFERENCES
