Stachys affinis tubers are known for its high content of stachyose and eaten as an edible vegetable. In this work, we assessed on the anti-inflammatory and anti-proliferation activity of a various type of extracts derived from S. affinis tubers. The n-hexane and dichloromethane fractions were showed the high cytotoxicity on the cell lines including RAW264.7 macrophages, HEK293 human kidney cell, A549 human lung cancer cell, KB human oral cancer cell, and a PC-3 human prostate cancer cell. N-butanol and water fractions were not exhibited cytotoxicity on the tested cancer cells, limited in anti-inflammatory and anti-cancer activities. Nevertheless, the ethyl acetate fraction showed little harm to RAW264.7 cells but inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) significantly. In addition, it arrests the cell growth in A549, KB, and PC-3 cell while little cytotoxicity on HEK293 cells. Consequently, these results supported that the ethyl acetate fraction of S. affinis tubers could be a potential anti-inflammatory and anti-cancer ingredient.

Abstract
 Introduction
 Materials and Methods
  Chemicals and reagents
  Extraction
  Cell line and culture
  Cytotoxicity on RAW 264.7 cells
  Determination of nitric oxide production of LPS-stimulatedRAW 264.7 cells
  Determination of prostaglandin E2 production of LPSstimulatedRAW 264.7 cells
  Anti-proliferation activity and cytotoxicity
  Statistical analysis
 Results
  Cytotoxicity of fractions from S. affinis tubers on RAW264.7cells
  Nitric oxide inhibition activity of fractions from S. affinistubers
  Inhibition of prostaglandins E2 production
  Cytotoxicity of fractions from S. affinis on HEK293 cells
  Anti-proliferation activity of fractions from S. affinis oncancer cells
 Discussion
 References

• Hui-Fang Guo(Department of Medical Biotechnology, Kangwon National University)
• Myeong-Hyeon Wang(Department of Medical Biotechnology, Kangwon National University) Corresponding author