This study was to investigate effect of tunicamycin (TM) on sperm viability, mitochondrial activity and motility in boar semen. Collected sperm were incubated with semen extender containing 0, 1, 2, and 5 μM TM for 3, 6 and 9 h. Sperm viability was analyzed using SYBR14/PI doubling staining, and mitochondrial activity was detected using Rhodamine123/PI staining methods. Sperm viability, mitochondrial activity and motility were measured every 3 h during incubation. In results, boar sperm viability, mitochondrial activity and motility were significantly decreased in 2 and 5 μM TM groups compare to control group at all incubation time (p<0.05). In addition, mitochondrial activity and motility were significantly decreased in 1, 2, and 5 μM TM groups compare to control group at 9 h after incubation (p<0.05). These results suggest that TM can inhibit sperm viability, mitochondrial activity and motility in boar semen, and it may influence on the fertility of sperm.

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 정액 준비
  2. 정자의 생존성
  3. 미토콘드리아 활성 검사
  4. 정자 운동성
  5. 통계처리
 Ⅲ. 결과
  1. Tunicamycin(TM)이 정자의 생존성의 미치는 영향
  2. 정자의 미토콘드리아 활성에 미치는 tunicamycin(TM)의 영향
  3. Tunicamycin(TM)이 정자 운동성에 미치는 영향
 Ⅳ. 고찰
 Ⅴ. 요약
 Ⅵ. 참고문헌

• 이아성(강원대학교 동물생명과학대학)
• 이상희(강원대학교 동물자원공동연구소) | Sang-Hee Lee (2Institute of Animal Resources, Kangwon National University)
• 이승형(강원대학교 동물생명과학대학) | Seunghyung Lee (Institute of Animal Resources, Kangwon National University) Corresponding author
• 양부근(강원대학교 동물생명과학대학) | Boo-Keun Yang (College of Animal Life Sciences, Kangwon National University)