It is noted that chalcone derivatives have characteristic diverse pharmacological properties, and that precise evidence has been growing that they could regulate a tumor necrosis factor-α (TNF-α) induced insulin resistance. The purpose of the present investigation is to elucidate the effects of the identified chalcone derivatives on adipogenesis, and to find the underlying mechanism of action in that case. Consequently, we first investigated whether the chalcone derivatives could affect the identified PPARγ-induced transcriptional activity on the proliferator-activated receptor response elements (PPRE) at target promoters, and find that trans-chalcone most significantly increased the PPARγ -induced transcriptional activity. Additionally, we confirmed that there were up-regulatory effects of trans-chalcone during the adipogenesis and lipid accumulation, and on the mRNA of adipogenic factors in 3T3-L1 cells. Next, we examined the effect of trans-chalcone on the inhibition induced by TNF-α on adipogenesis. To that end, we noted that the treatment with trans-chalcone attenuated the effect of TNF-α mediated secretion of various adipokines that are involved in insulin sensitivity. For this reason, we noted that this study clearly demonstrates that trans-chalcone enhanced adipogenesis, in part, by its potent effect on PPARγ activation and by its reverse effect on TNF-α.

서 론
 재료 및 방법
  세포 배양과 지방세포 분화 유도
  항체와 시약
  일시적 형질주입과 발광효소 분석법
  Oil red O 염색법
  mRNA 발현 분석
  통계분석
 결 과
  trans-chalcone은 PPARγ에 의한 전사활성을 더욱 증가시킨다
  trans-chalcone는 3T3-L1세포의 지방세포 분화 과정 동안 PPARγ 의 활성을 통해 지질 축적을 촉진한다
  trans-chalcone은 지방세포 분화과정 동안 adipogenic유전자의 발현을 증가시킨다
  trans-chalcone은 TNF-α의 지방세포 분화 억제를 반전시키는 효능을 갖는다
 고 찰
 Acknowledgements
 References

• Younho Han(Department of Oral Pharmacology, College of Dentistry, Wonkwang University, Iksan, Republic of Korea) Correspondence to