It is noted that Streptococcus mutans (S. mutans) triggers dental caries establishment by two major factors: the synthesis of organic acids, which demineralize dental enamel, and the synthesis of glucans, which mediate the attachment of bacteria to the tooth surface. Therefore, it is noted that the development of a more effective, substantial and safe preventive agent that works against dental caries and periodontal disease is required at this time. For this reason, the present study was designed to investigate the effect of croton seed ethanol extracts on the growth, acid production, adhesion, and insoluble glucan synthesis of S. mutans. In this case, the ethanol extract of croton seed showed concentration dependent inhibitory activity against the growth, acid production and adhesion of S. mutans. Especially, it is important to note that it has produced significant inhibition at the concentration of 0.1 and 0.2 mg/ml as compared to the control group. Moreover, these results suggest that the application of croton seed extract may be considered to be a useful method for the prevention of dental caries.

서 론
 재료 및 방법
  실험재료의 준비
  균주 및 배양
  S. mutans의 성장과 산생성 억제 검사
  S. mutans의 hydroxyapatite bead(HA) 부착 억제 검사
  Glucosyltransferase (GTFase)의 준비
  GTFase에 의한 비수용성 글루칸 합성 억제능 검사
  정성실험을 통한 파두의 성분 검사
  통계 분석
 결 과
  파두 에탄올 추출물의 S. mutans 성장에 관한 억제 효과
  파두 에탄올 추출물의 S. mutans 유기산 생성에 관한억제효과
  파두 에탄올 추출물의 S-HA 부착 억제에 관한 효과
  파두 에탄올 추출물의 GTFase에 의한 비수용성 글루칸 합성 저해활성에 관한 효과
  정성실험을 통한 파두의 성분 검사
 고 찰
 References

• Kang-Ju Kim(Department of Oral Microbiology, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, Republic of Korea) Correspondence to
• Yong-Ouk You(Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, Republic of Korea) Correspondence to
• Chung-Hoon Kang(Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, Republic of Korea)
• Sam-Sung Jung(Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, Republic of Korea)
• Ji-Hee Kim(Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, Republic of Korea)