This study was conducted to develop an agent-based computing platform enabling simulation of on-farm produce contamination by enteric foodborne pathogens, which is herein called PPMCS (Preharvest Produce Microbial Contamination Simulator). Also, fecal contamination of preharvest produce was simulated using PPMCS. Although Agent-based Modeling and Simulation, the tool applied in this study, is rather popular in where socio-economical human behaviors or ecological fate of animals in their niche are to be predicted, the incidence of on-farm produce contamination which are thought to be sporadic has never been simulated using this tool. The agents in PPMCS including crop, animal as a source of fecal contamination, and fly as a vector spreading the fecal contamination are given their intrinsic behaviors that are set to be executed at certain probability. Once all these agents are on-set following the intrinsic behavioral rules, consequences as the sum of all the behaviors in the system can be monitored real-time. When fecal contamination of preharvest produce was simulated in PPMCS as numbers of animals, flies, and initially contaminated plants change, the number of animals intruding cropping area affected most on the number of contaminated plants at harvest. For further application, the behaviors and variables of the agents are adjustable depending on user’s own scenario of interest. This feature allows PPMCS to be utilized in where different simulating conditions are tested.

Materials and Methods
  모의실험 대상 및 개체기반 프로그램 개발환경
  체기반 프로그램의 구동
  개발된 개체기반 프로그램을 이용한 모의실험
 Results
  작물 재배지역 출입 초식동물 개체수 영향 모의실험 결과
  작물 재배지역 서식 위생해충 개체수 영향 모의실험 결과
  초기 오염 작물 개체수 영향 모의실험 결과
 Discussion
 Acknowledgement
 국문요약
 References

• 류재기(농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀, Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) | Jae-Gee Ryu
• 김현주(농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀, Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) | Hyun-Ju Kim
• 김황용(농촌진흥청 기술협력국, Technology Cooperation Bureau, RDA) | Hwang-Yong Kim
• 양성규(성균관대학교 물리학과, Department of Physics, Sungkyunkwan University) | Seong-Gyu Yang
• 이기훈(농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀, Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) | Ki-Hoon Lee
• 한상현(농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀, Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) | Sanghyun Han Correspondence to