For people who have a food allergy the only way to manage the allergy is to avoid the food allergen. The mackerel is one of the major food allergens, but no immunoassay for the rapid and simple detection of mackerel has been reported. The objectives of this study are to develop and characterize monoclonal antibodies (MAbs) specific to mackerel using thermal stable-soluble proteins (TSSP) as an immunogen and to characterize the MAbs by indirect enzyme-linked immunosorbent assay (iELISA). The mice immunized with mackerel TSSP and showing high titer were used for cell fusion and cloning. The characterization of MAbs produced from hybridoma cells obtained was confirmed by indirect ELISA and western blot. Four MAbs were confirmed to be specific to mackerel without crossreaction to other marine products and livestock products in the both methods. The iELISA and western blot based on the MAbs can sensitively detect 1% mackerel protein in other marine products. These results support that immunochemical methods based on the MAb produced could be used as rapid means to detect low levels of mackerel and to identify mackerel adulterated in food.

Materials and Methods
 시료 및 실험재료
  TSSP의 추출
  Buffer에 따른 TSSP 추출효율 확인
  면역 항원 준비
  단클론성 항체 생산
  단클론성 항체 특성 확인
 Results and Discussion
  고등어 어육 중 TSSP의 확인
  TSSP 추출법 최적화
  단클론성 항체 생산 및 반응성 확인
  생산된 항체를 이용한 효소면역분석법과 western blot의검출한계 확인
 Acknowledgement
 국문요약
 References

• 이정은(경상대학교 응용생명과학부, Division of Applied Life Science, Graduate School, Gyeongsang National University) | Jeong-Eun Lee
• 김정숙(경상대학교 응용생명과학부, Division of Applied Life Science, Graduate School, Gyeongsang National University) | Jeong-Sook Kim
• 정덕화(경상대학교 농업생명과학연구원, 경상대학교 농화학식품공학과, Institute of Agriculture and Life Science, Gyeongsang National University, Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University) | Duck-Hwa Chung
• 심원보(경상대학교 농업생명과학연구원, 경상대학교 농화학식품공학과, Institute of Agriculture and Life Science, Gyeongsang National University, Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University) | Won-Bo Shim Correspondence to