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SCAR 마커 개발 및 이를 활용한 국내 육성 복숭아 품종 판별 KCI 등재

Identification of new Breeding Lines by Prunus Persica Cultivar-Specific SCAR Primers

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  • URLhttps://db.koreascholar.com/Article/Detail/34624
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한국육종학회지 (Korea Journal of Breeding Science)
한국육종학회 (The Korean Breeding Society)
초록

복숭아의 기원은 중국으로 알려져 있으며, 저장기간이 짧아 소비자들의 선호도가 낮았다. 이런 이유로 육종가들은 저장성을 높이는 것과 향과 맛을 좋게 하는데 육종 목표를 설정했으며, 국립원예특작과학원 육종가들은 이 목표에 따라 새로운 품종(‘천홍’, ‘수홍’, ‘하홍’, ‘유명’, ‘백미조생’, ‘천향’, ‘진미’, ‘수미’, ‘미스홍’, ‘유미’)을 육성하였다. 국립원예특작과학원 과수과에서는 육성 품종의 보호와 특허권을 확보하기 위해 분자생물학적 마커의

Peaches (Prunus persica) are less popular than the fresh fruits, because their flesh gets soft faster. So many breeders focused on their aim to firmness. Other breeders focused on juiciness, flavor and aroma. Breeding requires much labor, time and money. To reduce these requirements, many scientists develop many SSR, CAPS and SCAR makers. New peach varieties bred in our National Institute of Horticultural & Herbal Science (NIHHS) such as, Cheonhong, Suhong and Harhong are yellow flesh cultivars and Yumyeong, Baekmijosaeng, Baekhyang, Jinmi, Soomee, Mihong, Misshong and Yumee are white flesh cultivars. These peach cultivars are planted in orchard of Korea. To assert breeding cultivar patents and prevent patent disputes, we detected cultivar-specific DNA fragment using 235 sets of Operon RAPD primers, analyzed 134 DNA sequences and constructed SCAR primers. To confirm the cultivar-specific SCAR markers, we applied candidate SCAR primers to 30 peach cultivars widely cultivated in Korea. These selected lines are included father and mother lines that were used to develop new varieties in NIHHS. Using fourteen SCAR primer sets, we characterized thirty cultivars selected. The SCAR marker is expected to serve as molecular evidence distinguishing different peach varieties.

저자
  • 한상은(국립원예특작과학원) | Sang Eun Han
  • 조강희(국립원예특작과학원) | Kang Hee Cho
  • 남은영(국립원예특작과학원) | Eun Young Nam
  • 신일섭(국립원예특작과학원) | Il Sheob Shin
  • 김정희(국립원예특작과학원) | Chung Hee Kim
  • 김현란(국립원예특작과학원) | Hyun Ran Kim
  • 김대현(국립원예특작과학원) | Dae Hyun Kim