An efficient method for in vitro propagation and growth of the wild garlic(Allium ochotense Prokh.) was established. Bulbs of wild garlic were collected from Ullung Island, Korea, and the growth pattern of plantlets on various culture media was observed. High growth of shoot was obtained on LP, NN and N6 medium. The growth media supplemented with 3%(w/v) sucrose was found optimal for shoot growth. After 10 weeks multiple shoots were observed in the plantlets growing on the medium containing 1.0 mg/l of zeatine and 0.1 mg/l NAA. Roots were induced directly at the base of the shoot in all treatments. The medium with 2.0 mg/l of IBA proved to be the best rooting medium. The studies of this kind may be used to develop strategies for large-scale propagation of elite wild garlic.

ABSTRACT
 Introduction
 Materials and methods
  1 Plant material and in vitro culture
  2 Determination of optimal culture media
  3 Shoot proliferation through shoot tip
  4 Shoot elongation and in vitro rooting
  5 Acclimatization of in vitro plantlets
  6 Statistical analysis
 Results and discussion
  1 Effect of culture media on shoot growth
  2 Effect of various carbon sources on plant growth
  3 Shoot proliferation of wild garlic through shoot tip
  4 In vitro rooting and acclimation
 References

• Cheul Ho Lee(Plant Conservation Division, Korea National Arboretum)
• Mi Jin Jeong(Plant Conservation Division, Korea National Arboretum) Corresponding author