In this study, a nonlinear analysis method was proposed to investigate serviceability of rail fastening system in concrete track at the railway bridge end. The serviceability investigation method of the fastening system in concrete track is time consuming and is complex in design because of the many decks and fastening systems. In addition, there is also a case where an expensive special fastening device is installed due to the stiffness of the rail fastening device assumed to be linear and the excessive design result by the linear analysis method. Therefore, in this study, the clamping force test was performed to confirm the nonlinearity of fastener. The conventional linear analysis method and the proposed nonlinear analysis method were performed for the railway bridge model specimen with concrete tracks, and the uplift forces acting on the fastening systems were compared and analyzed.