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Sprague-Dawley Rats을 이용한 결명자 물 추출물의 26주 반복 경구투여 독성시험 및 4주 회복시험 KCI 등재
A 26-Week Repeated Oral Dose Toxicity Test and a 4-Week Recovery Test of Cassia tora L. Water Extract in Sprague-Dawley Rats
pp.157-169
Background: Cassia tora L., an annual or perennial plant of the Fabaceae family, is traditional medicine with various biological activities, including anti-constipation and, anti-inflammation. Chemical compounds such as anthraquinone glycoside and naphthalene derivatives have been isolated from this plant. Cassia tora L. is a common contaminant of agricultural commodities, but is toxic to cattle and poultry.
Methods and Results: To investigate the potential toxicity, Cassia tora L. aqueous extract (CO) was administered orally to rats for 26 weeks at 0 (control), 300, 1,500 and 3,000㎎/㎏/day (n = 10 for male rats for each dose). The positive control comprised animals orally administered anthraquinone 100㎎/㎏/day. There was no treatment-related mortality. An increase in the kidney weight was observed at 3,000㎎/㎏/day of CO and anthraquinone 100㎎/㎏/day. Macrophage infiltration in the colon was observed at CO 1,500 and 3,000㎎/㎏/day and anthraquinone 100㎎/㎏/day, but there were no significant toxicological changes in the incidence and severity of the finding.
Conclusions: The oral no-observed-adverse-effect level (NOAEL) of CO was 3,000㎎/㎏/day in male rats and no target organs were identified. In addition, 300㎎/㎏ was found to be the no-observed-effect level (NOEL) for systemic toxicity under the conditions of the study.
서 언
재료 및 방법
1. 실험 재료, 추출물의 제조 및 투여
2.실험동물
3.관찰 및 검사항목
4. 통계처리
결 과
1. 일반 증상
2.체중 변화
3. 사료 섭취량
4.뇨 검사
5. 혈액학적 검사
6. 혈액생화학적 검사
7. 정자검사
8. 부검
9.장기중량
10.조직병리학적 검사
고 찰
REFERENCES
