Background: To obtain useful cosmetic resources, this study aimed to determine the non-saponin fatty acid and inhibitory activities of collagenase and elastase by treatment of Saccharomyces cerevisiae in supercritical fluid extracted oil of the adventitious root culture of wild mountain ginseng.
Methods and Results: We performed supercritical fluid extraction at various conditions such as pressure, temperature, time, and use of co-solvents, unlike the n-hexane extraction for the adventitious roots culture of wild mountain ginseng. The non-saponin-fatty acid obtained from the oil of the adventitious roots culture was incresed by treatment with S. cerevisiae. The supercritical fluid extraction was conducted using gas chromatography. Non-saponin-fatty acid content, in the oil of adventitious roots culture of wild mountain ginseng treated with S. cerevisiae for 2 days were three times higher than that in the control. In addition, the oil of the adventitious roots culture treated with S. cerevisiae was investigated for the anti-wrinkle effect by using collagenase and elastase. The oil of adventitious roots culture treated with S. cerevisiae exhibited higher collagenase and elastase inhibitory activities than those in the control.
Conclusions: Supercritical fluid extracted oil of the adventitious roots culture of wild mountain ginseng treated with S. cerevisiae was found to have decreased ratio of saturated fatty acids and incresed ratio and content of unsaturated fatty acids increased. Furthermore, it showed anti-wrinkle effects in vitro.

ABSTRACT
 서 언
 재료 및 방법
  1. 재료
  2. 기기 및 시약
  3. 지방산 초임계 유체 추출조건 및 수율
  4. 산삼 부정 배양근 발효미생물 처리
  5. 지방산 분석
  6. 계층적 군집분석
  7. Elastase 저해활성 측정
  8. Collagenase 저해활성 측정
  9. 통계처리
 결과 및 고찰
  1. 지방산 초임계 유체 추출조건 및 수율
  2. 지방산 분석
  3. 계층적 군집분석
  4. Elastase 저해활성
  5. Collagenase 저해활성
 REFERENCES

• 김철중(㈜화진바이오코스메틱) | Chul Joong Kim (Research Institute of Biotechnology, HwajinBioCosmetic)
• 심재권(㈜화진바이오코스메틱) | Jae Kwon Shim (Research Institute of Biotechnology, HwajinBioCosmetic)
• 권경철(㈜화진바이오코스메틱) | Kyung Cheol Kwon (Research Institute of Biotechnology, HwajinBioCosmetic)
• 임정대(강원대학교 생약자원개발학과) | Jung Dae Lim (Department of Herbal Medicine Resource, Kangwon National University)
• 최선강(강원대학교 농생명산업학과) | Seon Kang Choi (Department of Agricultural Life Sciences, Kangwon National University)
• 유창연(강원대학교 생물자원과학과) | Chang Yeon Yu (Department of Bio-Resource Sciences, Kangwon National University)
• 이재근(㈜화진바이오코스메틱) | Jae Geun Lee (Research Institute of Biotechnology, HwajinBioCosmetic) Corresponding author