Because of the characteristics of long-span bridges, the cable tension is considered a critical item in their maintenance. In particular, because the evaluation of the cable tension in a suspension bridge is essential for understanding the general status of the structural system, identifying the initial values of this force in the construction of a bridge and then accurately predicting and comparing its estimated values during traffic use are very important tasks for the maintenance of a suspension bridge. In this paper, to verify the algorithm for estimating the cable tension of hanger cables using the back analysis technique, an ambient vibration test was conducted at a suspension bridge.