In this study, effects of nutrient and inorganic carbon on single cell emergence during the cultivation of microalgae were observed using colonial green algae, Pediastrum duplex. The concentration of inorganic carbon had significant effect on single cell emergence and its growth, but nitrogen and phosphorus concentration showed minor effects. According to P. duplex cultivation experiment, single cell started to be emerged around 500~750 mg-C/L of inorganic carbon concentration and it was bloomed dramatically at the higher values. And growth of P. duplex was started to be surpressed at the single cell formation concentration. From the results, it could be said that when we operate the microalgae systems for cultivation/harvesting or wastewater treatment, in order to avoid single cell formation, inorganic carbon should be maintained to the proper level

ABSTRACT
 1. 서 론
 2. 연구방법
  2.1 P. duplex의 분리 및 배양
  2.2 반응조 구성
  2.3 실험방법
  2.4 P. duplex의 성장량 및 single cell 발생량 분석방법
 3. 결과 및 고찰
  3.1 질소, 인 및 무기탄소 농도에 따른 single cell 발생량
  3.2 P. duplex의 single cell이 출현되는 무기탄소농도
 4. 결 론
 사 사
 References

• 조재형(경희대학교 공과대학 환경학 및 환경공학과, Department of Environmental Science and Engineering, Kyung Hee University) | Jae-Hyung Cho
• 노경호(경희대학교 공과대학 환경학 및 환경공학과, Department of Environmental Science and Engineering, Kyung Hee University) | Kyung-Ho Noh
• 박규현(경희대학교 공과대학 환경학 및 환경공학과, Department of Environmental Science and Engineering, Kyung Hee University) | Kyu-Hyun Park
• 장중석(한국농어촌공사 농어촌연구원, Korea Rural Community Corporation) | Jung-Seok Jang
• 남귀숙(한국농어촌공사 농어촌연구원, Korea Rural Community Corporation) | Gui-Sook Nam
• 황선진(경희대학교 공과대학 환경학 및 환경공학과, Department of Environmental Science and Engineering, Kyung Hee University) | Sun-Jin Hwang Corresponding author