The virus-like particle (VLP) is similar to a pathogenic virus and has a high immunogenicity. However, in the selection of various structural proteins to form VLP, all expression systems commonly consume most of the time and suffer from various difficulties. Therefore, there is a need for a system that can rapidly determine the structural proteins required for effective VLP production. This study aims to construct a transient expression platform using insect cells to solve this problem. Plasmid-based expression vectors and baculovirus-inducible expression vectors were constructed. The vectors were evaluated for overexpression using EGFP. We also confirmed the formation of virus like particles through overexpression of virus structural proteins.

• Won Seok Gwak(Department of Agricultural Biology, Chungbuk National University)
• Hyun Soo Kim(Department of Agricultural Biology, Chungbuk National University)
• Soo Dong Woo(Department of Agricultural Biology, Chungbuk National University)