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Development of SCAR Marker for Identification of the Perilla Species KCI 등재

Myoung Hee Lee, Ki Woong Yang, Tae Joung Ha, Chan Sik Jung, Suk Bok Pae, Chung Dong Hwang, Chang Hwan Park, In Youl Baek, Hyeon Kyeong Kim, Soon Ki Park
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  • URLhttps://db.koreascholar.com/Article/Detail/34692
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한국육종학회지 (Korea Journal of Breeding Science)
한국육종학회 (The Korean Breeding Society)
초록

This study is to generate SCARs markers for identification of Perilla species. A SCAR is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. We derived SCARs by sequencing and cloning the both ends of the amplified products of RAPD markers. Sixteen sequence-specific primers were synthesized from eight RAPD markers, which were completely sequenced. We developed the species-specific SCAR markers which could be used successfully in detecting genetic variation in four Perilla species. These markers could be used to verify species-origins of various forms of Perilla germplasms.

키워드
Perilla frutescensMolecular markerRandom Amplification of Polymorphic DNA (RAPD)Sequence Characterized Amplified Regions (SCARs)
저자
  • Myoung Hee Lee
  • Ki Woong Yang
  • Tae Joung Ha
  • Chan Sik Jung
  • Suk Bok Pae
  • Chung Dong Hwang
  • Chang Hwan Park
  • In Youl Baek
  • Hyeon Kyeong Kim
  • Soon Ki Park