논문 상세보기
pp.225-231
1. 선행 연구에서 염색체 3번의 RM60~RM231 부근에서 종자중, 수당립수에 관여하는 QTL이 탐지되었고, 이를 확인하기 위하여 이 지역에 크기와 위치가 다른 O. glaberrima 단편이 이입된 5 계통을 선발하여 표현형을 조사하였다. 실험 결과 계통별로 출수기, 임실률, 수당립수, 종자중을 제외한 형태적 특성들이 밀양23호와 비슷한 양상을 보였다. 이는 대부분의 염색체 지역이 밀양23호로 회복되었기 때문이라고 보여진다. 2. 유전자의 위치를
In previous studies, we reported QTLs for grain weight (GW), qGW3 and for spikelets per panicle (SPP), qSPP3 linked to RM60 on chromosome 3 using advanced backcross lines derived from a cross between Oryza sativa ssp. Indica cv. Milyang 23 and O. glaberrima. The O. glaberrima alleles at this locus increased GW and spikelets per panicle in the Milyang 23 background. To further confirm and narrow down the position of the QTLs on chromosome 3, substitution mapping was performed using five lines containing the target O. glaberrima segment on chromosome 3. The size and position of the O. glaberrima segment on chromosome 3 were different in each line. These lines possessed 3-10 non-target O. glaberrima introgressions in the Milyang 23 background. These five lines were evaluated for seven agronomic traits including 1,000 grain weight and spikelets per panicle and also genotyped with seven SSR markers. Four lines were informative in delimiting the position of QTLs, qGW3 and qSPP3. Two lines with the O. glaberrima segment flanked by SSR markers, RM60 and RM523 displayed significantly higher values than Milyang 23 in GW and SPP whereas two lines without that O. glaberrima segment displayed no difference in GW and SPP compared to Milyang 23. The result indicates that two QTL, qGW3 and qSPP3 are located in the interval between RM60 and RM523 which are 1.2-Mb apart. Introgression lines having QTLs, qGW3 and qSPP3 would be useful materials not only to indentify the relationship between these two yield QTLs, but also to develop high yielding variety via marker-aided selection technology.
