In this study, we developed 12 microsatellite markers specific to N. pygmaea using Illumina paired-end sequencing. Forty individuals of N. pygmaea collected from three currently known localities in South Korea were genotyped to validate these markers and to preliminarily assess population genetic characteristics. No locus showed significant deviation from the Hardy–Weinberg equilibrium (HWE). Our preliminary data indicate an absence of inbreeding in all populations and an absence of obvious genetic difference. The microsatellite markers developed in this study will be useful for studying the population genetics of N. pygmaea collected from other regions, including additional sites in South Korea.

• Min Jee Kim(Department of Applied Biology, Chonnam National University, Gwangju)
• Ah Rha Wang(Department of Applied Biology, Chonnam National University, Gwangju)
• Sung Soo Kim(Research Institute for East Asian Environment and Biology)
• Junghwa An(Animal Resources Division, National Institute of Biological Resources)
• Iksoo Kim(Department of Applied Biology, Chonnam National University, Gwangju)