Mediterranean fruit fly, Ceratitis capitata, is one of the most important quarantine pest worldwide. Fruit flies cause serious damage on orchard, and also can cause quarantine problem to fruit exportation. Recently, because of global warming, there is threats of fruit fly invasion to Korea, especially to Jeju island. So there should be a anticipative research about fruit fly control. Many physical quarantine treatment methods, like heat treatment and low-temperature treatment, are developed to control quarantine pests. Recent researches indicate that low-temperature treatment shows good efficacy on several quarantine pests without phytotoxicity. In this trial, we’ve applied the low-temperature treatment on citrus at laboratory and pilot scale containers to validate efficacy and phytotoxicity. Egg and larvae stage of C. capitata was completely controlled after 5 days and 8 days low-temperature treatment at 0℃ respectively, and there was no phytotoxicity on citrus. These results indicate that the low-temperature treatment can be applied to control fruit fly on citrus fruit.