The effects of cold storage temperature and exposure duration on immature stages of spotted-wing drosophila (SWD) Drosophila suzukii (Diptera: Drosophilidae) on ‘Campbell Early’ grapes were examined to establish a phytosanitary control method. The immature stages (eggs, larvae and pupae) of SWD were all dead after a 6-day cold treatment at 1°C and 8-day cold treatment at 1.5 and 2°C. Small-scale tests using pupae, which were the most-cold tolerant stage, confirmed the validity of the selected temperature and exposure durations. Conversely, the 8- and 10-day at 1°C treatments showed 100% mortality, suggesting that these treatments can guarantee quarantine security against infestations of SWD on exported ‘Campbell Early’ grapes.

• Min Jee Kim(Department of Applied Biology, Chonnam National University)
• Jong Seok Kim(Department of Applied Biology, Chonnam National University)
• Jun Seong Jeong(Department of Applied Biology, Chonnam National University)
• Deuk-Soo Choi(Department of Plant Quarantine, Animal and Plant Quarantine Agency)
• Jinyoung Park(Department of Ecological Monitoring and Assessment, National Institute of Ecology)
• Iksoo Kim(Department of Applied Biology, Chonnam National University)