The susceptibility of Dermanyssus gallinae adults, collected from poultry farms in 5 regions, to 11 acaricides was investigated. When bifenthrin and formic acid with pyridaben were diluted 100 times, bifenthrin showed 100% acaricidal activity; however, formic acid with pyridaben showed less than 20% acaricidal activity for poultry farms in 3 regions (Gyeongju, Chilgok, and Geumsan) except Yeoncheon and Anseong. Clothianidin, thiamethoxam, fenitrothion, and formic acid with pyridaben showed differences in acaricidal activity among regions. Therefore, farmers should concentrate during the selection of these acaricides. However, carbaryl, cartap hydrochloride, dichlorvos, and bifenthrin showed high activity against D. gallinae collected from poultry farms in five regions. To control D. gallinae effectively, the alternation of acaricides is necessary, and indiscriminate pesticide use should be avoided. Therefore, this study can serve as a basis for controlling D. gallinae.