Highly pathogenic avian influenza virus (HPAIV) is already panzootic in poultry and caused a considerable economic loss in poultry industry. In addition, HPAIV continues to cross species barriers to infect humans and other mammals, often with fatal outcomes. In this study, the virucidal efficacy of Citra-Kill® composed to quaternary ammonium chloride and citric acid was investigated against avian influenza H9N2 virus (AIV). A virucidal efficacy was determined with the viability of AIV contacted with the disinfectant in the allantoic membrane of chicken embryos. Citra-Kill® and AIV was reacted on the distilled water (DW), hard water (HW) or organic matter suspension (OM) condition. On DW condition, AIV was inactivated with 2,000 fold dilutions of Citra-Kill®. When the antiviral effect on HW condition was evaluated, the antiviral activity of the disinfectant showed on 1,500 fold dilutions against AIV. With the investigation of the antiviral effect of the disinfectant on OM condition, AIV was inactivated on 500 fold dilutions of Citra-Kill®. As Citra-Kill® possesses virucidal efficacy against AIV, the disinfectant solution can be used to limit the spread of animal viral diseases.

ABSTRACT
 Introduction
 Materials and methods
  Disinfectant
  Avian influenza virus and culture
  Diluents and treatment condition
  Virus-disinfectant contact reaction
  Evaluation of Citra-Kill® against AIV
 Results and Discussion
 Acknowledgements
 요 약
 References

• Chun-Nam Cha(Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
• Yeo-Eun Lee(Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University)
• In-Jin Kang(Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University)
• Chang-Yeul Yoo(Department of Computer Information, Gyeongnam Provincial Namhae College)
• Eun-Kee Park(Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University)
• Sunjeong An(Department of Occupational Therapy, Inje University)
• Suk Kim(Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University)
• Hu-Jang Lee(Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University) Correspondence to