This study was designed to evaluate a repeated oral dose toxicity and immunomodulating activity of Pulsatilla koreana and Artemisiae annuae in Sprague-Dawley rats. The female rats were treated with Pulsatilla koreana and Artemisiae annuae of control group, low group (0.5 ml/kg), medium group (1 ml/kg), high group (2 ml/ kg) for distilled water, intragastrically for 4 weeks, respectively. To ensure the safety of Pulsatilla koreana and Artemisiae annuae such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. Hematological results were the increase of neutrophils, lymphocytes and monocytes in the high dose group of Pulsatilla koreana. The increase immune cells in the high dose group of Pulsatilla koreana might immunomodulating activity. No significant differences in body weight, feed intake, serum biochemical analysis and histopathological between control and fed group were found. In conclusion, Pulsatilla koreana and Artemisiae annuae is physiologically safe and improve immunomodulating activity.

ABSTRACT
 서 론
 재료 및 방법
  시험물질
  실험동물
  시험군의 구성, 투여용량 및 용량
  관찰 및 검사항목
 결과 및 고찰
  임상증상
  체중변화
  사료 및 음수섭취량 변화
  안과학적 검사소견
  혈액학적 검사소견
  혈청 생화학적 검사소견
  체중에 대한 각 장기의 상대중량비
  병리조직검사 소견
 요 약
 감사의 글
 참고문헌

• 안인정(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | In-Jung An
• 권중기(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Jung-Ki Kwon
• 이진석(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Jin-Seok Lee
• 이성호(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Seung-Ho Lee
• 박영석(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Young-Seok Park
• 박병권(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Byung-Kwon Park
• 김상기(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Sang-Ki Kim
• 김병수(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Byeong-Soo Kim
• 조성대(전북대학교 구강병리학교실, School of dentistry, Institute of Oral Bioscience, Chonbuk National University) | Sung-Dae Cho
• 최창순(중앙대학교 식품공학부, School of Food Science and Technology, Chung-Ang University) | Chang-sun Choi
• 이병희(국립생물자원관, National Institute of Biological Resources) | Byoung-Hee Lee
• 강병곤((주)신일바이오젠, Shinil Biogen) | Byeong-Kon Kang
• 정지윤(공주대학교 두과녹비자원센터, Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University) | Ji-Youn Jung Correspondence to