This study intented to standardize the method for total polysaccharide, which is a functional marker for aloe vera gel in Korea. We used four lyophilized raw materials and commercial aloe gel products, certified as Health Functional Food by Korea Food and Drug Administration, including powder, solution, jelly, tablet and capsule, to optimize the analytical condition of dialysis and phenol-sulfuric acid reaction in polysaccharide analysis. The optimal conditions for polysaccharide analysis included 1 L water for dialysis and change 3 times for 24hr against 25 mL prepared sample solution. Validation test showed lower than 5% of coefficient of variation(CV) in intra-, interday validation in lyophilized raw materials and 4 types of commercial products. In inter-person and inter-laboratory validation with 4 persons from 4 different laboratories, CV(%) were 5.50 and 6.64 respectively. The linearity of polysaccharide analysis was assessed using 5 serial concentration of lyophilized raw materials(0.1, 0.2, 0.3, 0.4, 0.5%(w/v)). The results showed R2 ≥ 0.995 of high linearity. In the commercial aloe vera gel products, the results of reproductivity showed lower than 7.08% and revealed that the standardized method from this study ensured high precision for polysaccharide analysis.

ABSTRACT
 재료 및 방법
  시료
  장비와 시약
  표준용액 조제
  시료전처리 및 시험용액 제조
  발색 및 흡광도 측정
  시험법 검증
  유통제품 분석
 결과 및 고찰
  투석의 최적 조건 설정
   투석막 규격 비교
   투석후 정용 여부의 비교
  페놀황산 반응 조건에 따른 정밀도
  시험법 표준화에 따른 시험법 검증
   일내 일간 정밀성 분석
   실험자간·실험실간 정밀성 분석
  직선성 확인
  유통 제품의 제형별 정밀성
 요 약
 감사의 글
 참고문헌

• 이영주(식품의약품안전평가원 영양기능연구팀, Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) | Young Joo Lee
• 김윤제(식품의약품안전평가원 영양기능연구팀, Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) | Yunje Kim
• 임동길(식품의약품안전평가원 영양기능연구팀, Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) | Donggil Leem
• 윤태형(식품의약품안전평가원 영양기능연구팀, Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) | Taehyung Yoon
• 신지은(식품의약품안전평가원 영양기능연구팀, Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) | Jieun Shin
• 윤창용(식품의약품안전평가원 영양기능연구팀, Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) | Changyong Yoon
• 김정훈(식품의약품안전평가원 영양기능연구팀, Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) | Junghoon Kim
• 박미선(식품의약품안전평가원 위해분석연구과, Department of Risk Assessment, National Institute of Food and Drug Safety Evaluation) | Misun Park
• 강태석(식품의약품안전평가원 영양기능연구팀, Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) | Tae Seok Kang
• 정자영(식품의약품안전평가원 독성연구과, Department of Toxicological Research, National Institute of Food and Drug Safety Evaluation) | Jayoung Jeong Correspondence to