Vibrio spp. and Streptococcus spp. have caused a considerable disease of farmed fish and economic loss in fish farming and seafood industry. In this study, the efficacy of an aquatic disinfectant tablet composed to calcium hypochlorite was evaluated against V. anguillarum and S. iniae. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4oC. An aquatic disinfectant tablet and test bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. V. anguillarum on the DW, HW and OM condition was completely inactivated with 16,000 15,000 and 13,000 fold dilutions of the disinfectant, respectively. On the DW, HW and OM condition, S. iniae was absolutely inactivated with 17,000 16,000 and 14,000 fold dilutions of the disinfectant, respectively. As an aquatic disinfectant tablet possesses bactericidal efficacy against fish pathogenic bacteria such as V. anguillarum and S. iniae this disinfectant solution can be used to control the spread of fish infective bacterial diseases.

ABSTRACT
 Materials and Methods
  Bacteria and culture
  Disinfectant
  Diluents and treatment condition
  Experimental procedures
 Results
 Discussion
 Conclusions
 Acknowledgements
 References

• Chun-Nam Cha(Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
• Yeo-Eun Lee(Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University)
• In-Jin Kang(Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University)
• Chang-Yeul Yoo(Department of Computer Information, Gyeongnam Provincial Namhae College)
• Hyunju Choi(Elderly Life Redesign Institute, Department of Biomedical Laboratory Science, Inje University)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Hu-Jang Lee(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Correspondence to