In this study, ribulose bisphosphate carboxylase (rbcL), RNApolymeraseC (rpoC1), intergenic spacer (psbA-trnH), and second internal transcribed spacer (ITS2) as identification markers for discrimination of P. mirifica in foods were selected. To be primer design, we obtained 719 bp, 520 bp, 348 bp, and 507 bp amplicon using universal primers from selected regions of P. mirifica. The regions of rbcL, rpoC1, and psbA-trnH were not proper for design primers because of high homology about P. mirifica, P. lobata, and B. superba. But, we had designed 4 pairs of oligonucleotide primers from ITS2 gene. Predicted amplicon from P. mirifica were obtained 137 bp and 216 bp using finally designed primers SFI12-miri-6F/SFI12-miri-7R and SFI12-miri-6F/SFI12-miri-8R, respectively. The species-specific primers distinguished P. mirifica from related species were able to apply food materials and processed foods. The developed PCR method would be applicable to food safety management for illegally distributed products in markets and internet shopping malls.

ABSTRACT
 재료 및 방법
  재료선정
  시약 및 검체 구입
  유전자 추출
  일반 프라이머를 위한 PCR 반응액 조성 및 반응조건
  종 특이 프라이머를 위한 PCR 반응액 조성 및 반응조건
  염기서열 분석
  PCR 산물의 확인
 결 과
  일반 프라이머를 이용한 PCR 및 염기서열 결정
  P. mirifica 판별을 위한 종 특이 프라이머 개발
  종 특이 프라이머를 이용한 가공식품의 적용성 검토
 고 찰
 요 약
 감사의 말
 참고문헌

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• 이재황(식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀, Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) | Jae-Hwang Lee
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