This study aimed to provide fundamental data for enlargement strategy of Ramsar site through the analysis of designation properties about 22 sites which has been registered for 20 years since Yongneup (Mt. Daeam) was designated as the first Ramsar site in Korea. Designation properties were analyzed on the basis of Ramsar Information Sheet and ecological properties was mainly analyzed in relation to designation criteria. Designation of Ramsar wetland was concentrated before and after Changwon Congress in 2008 and in terms of area 11 sites are below 1 ㎢ and 5 sites are above 10 ㎢. The average number of applied designation criteria was 2.86 and Criterion 2 (threaten species), Criterion 1 (representativeness and rareness of wetland type) and Criterion 3 (biodiversity) are mainly applied. In terms of protection species, internationally recognized species were 18 species on IUCN redlist, 12 species on CITES and 13 species on CMS, and nationally recognized species were 76 species on endangered species designated by Ministry of Envirionment, 83 speceis on national redlist and 71 speceis on endemic species respectively