Mushroom Research Division, National Institute of Horticultural and Herbal Science (NIHHS) has operated mushroom resource management facilities which consist of eight separate rooms with automatic temperature and humidity controllers for the safe preservation of mycelial cultures and dried voucher specimens. Recently the liquid-nitrogen (LN) cryogenic system for the permanent/semi-permanent preservation of the strains commercially or scientifically important are successfully installed. Currently, 3,072 strains of 493 species in 139 genera of edible and medicinal mushrooms are preserved. During the last two years, an intranet site was designed and constructed for efficiently managing genetic resources. Based on accumulated data, morphological and physiological characteristics of mycelia and fruiting bodies, genetic sequences such as DNA barcodes and other molecular markers, preservation information, and the related pictures and documents of each strain are provided via graphical user interface.