In this study, we made population with high biological efficiency to investigate the complex genetic architecture of yield-related traits in A. bisporus. MB013 that crossed with bisp 15-p2 and bisp 34-p2 had high biological efficiency. 170 homokaryons was isolated with CAPS marker (PIN primer/HaeⅢ) from 1000 ISSs. And 100 BC1F1 hybrids obtained by crossing the homokaryons of MB013 with bisp15-p1. Parental line bisp 15-p2 and bisp 34-p2 and 100 homokaryons of MB013 will analyze genome sequencing. Also 100 BC1F1 hybrids will evaluate yield-related traits.

• Youn-Lee Oh(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
• Pyung-Gyun Shin(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
• Minji Oh(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
• Ji-Hoon Im(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
• Won-sik Kong(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
• Kab-Yeul Jang(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)