Occurrence spontaneous mushrooms in eastern Jeju Muryeongarioreum, Sillyecheon-Iseungak, Kyoraegotjawal, Geomunoreum were examined in 2016. The collected mushrooms were classified by 15 order 56 family 149 genera 317 species. Among the collected mushrooms, 87 species of mushrooms were successfully incubated in mushroom complete medium in which DNA of 25 species were completely extracted and amplified using universal primers ITS1 (5’-CTTG GTCA TTTA GAGG AAGT AA-3’) and ITS4 (5’-TCCT CCGC TTAT TGAT ATGC-3’). Four species of them were differently identified between the classification by morphological observation and by analysis of DNA sequences i.e. by morphological identification Ganoderma applanatum, Armillaria gallica, Macrolepiota procera and Trametes gibbosa were identified as G. gibbosum, A. cepistipes, M. detersa and T. elegans by analysis of DNA sequences, respectively. Based on these results both identification ways might be necessary for correct classification of mushrooms. On the other hand, among the collected 317 mushrooms, 48 species could be identified to genus but not to species by the morphological observations. However, by the analysis of DNA sequences, 15 species were identified to species in which 6 species were unregistered mushrooms in Korea. Therefore, the analysis of DNA sequence may be useful to identify the mushrooms which are difficult to recognize by morphological observation.

• Seung Hak Lee(Faculty of Bioscience and Industry, College of Applied Life Sciences, The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University)
• Pyung Yeol Ko(Faculty of Bioscience and Industry, College of Applied Life Sciences, The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University)
• Yong Chull Jeun(Faculty of Bioscience and Industry, College of Applied Life Sciences, The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University)